Research Technician

We are seeking a highly motivated individual for a full-time Research Specialist position in Dr. Hun-Way Hwang’s laboratory in the Department of Pathology. Our current research focuses on the role of abnormal mRNA processing in human disease. We combine powerful in vivo high-throughput profiling methods and CRISPR technology with genetically modified mouse models of human disease to identify candidate mRNA isoforms that may contribute to disease pathogenesis in specific cell types in vivo. These candidate mRNA isoforms will then be further studied by in vitro and in vivo methods to reveal their biological functions and the molecular mechanisms regulating their processing, which will provide novel insights into the management and treatment of human disease. Our two recent publications describe how cancer cells switch mRNA isoforms to survive chemotherapy (https://doi.org/10.1016/j.celrep.2021.109815) and how hyperactive mTORC1 alters mRNA isoform expression in tuberous sclerosis complex (https://elifesciences.org/articles/85036). Additional information can be found at https://www.hwangrnalab.org.Applicants should have a Bachelor’s degree in Biology or related field. Significant undergraduate lab experience beyond coursework and familiarity with the basics of molecular biology and mammalian cell culture is desired.

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Maintain daily operation of the lab and mouse colonies. This will include:- Preparing common laboratory reagents- Ordering; filing inventories; tracking ongoing projects- Equipment maintenance- Lab organization to comply with chemical, radiation, and biohazard standards- Regular visits to the mouse facility, breeding, weaning and genotyping- Perform mammalian cell culture and molecular biology experiments including cell passage and maintenance, cell transfection, nucleic acid extraction, and conventional and real-time PCR

This position is primarily sedentary. Incumbent may be required to travel back and forth between laboratory areas with occasional lifting of reagent containers and placing buffers and solutions on shelves.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.